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Fully Automated Method Using Dispersive Liquid-Liquid MicroExtraction (DiLLME) for Extractable and Leachable Studies

Application Note

Dispersive Liquid-Liquid MicroExtraction (DiLLME) is a technique used to extract analytes from a product matrix and provides a high level of enrichment for trace analysis testing. This enables the use of smaller sample sizes whilst maintaining the low limits of detection often required. A completely automated solution using fast agitation and centrifugation has been developed on the GERSTEL MultiPurpose Sampler (MPS). The applicability of this method to the analysis of 11 analytes in a biopharmaceutical drug is demonstrated. The method showed good precision (n=6), recoveries (three spike levels) and linearity with RSDs of less 10%, recoveries between 90 and 110% and R2 greater than 0.995 (9 out of 11 analytes). This automated approach offers significant advantages over manual procedures in terms of analyst time, solvent usage and data quality.

Application Note Downloads

Fully Automated Method Using Dispersive Liquid-Liquid MicroExtraction (DiLLME) for Extractable and Leachable Studies


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